郭初阳,李冲,张海亮

• 1:咸阳市中心医院肿瘤治疗中心一病区
• 2:咸阳市中心医院普外科
• 3:咸阳市中心医院肿瘤治疗中心二病区

摘要(Abstract)：

目的:探究微小RNA-340(miR-340)对胃癌细胞增殖、自噬和黏附的影响及磷脂酰肌醇3-激酶(PI3K)/丝苏氨酸蛋白激酶(AKT)信号通路的调控作用。方法:将体外培养的人胃癌AGS细胞系分为对照组(Con组；不做干预)、空载质粒(mimics NC)组(转染mimics NC至AGS细胞)、miR-340过表达质粒(miR-340 mimics)组(转染miR-340 mimics至AGS细胞)、miR-340 mimics+抑制剂组(转染miR-340 mimics至AGS细胞后加10μmol/L PI3K/AKT通路抑制剂LY294002)和miR-340 mimics+激活剂组[转染miR-340 mimics至AGS细胞后加50mg/ml PI3K/AKT通路激动剂胰岛素样生长因子(IGF)-Ⅰ],转染成功后药物干预24h。miR-340的表达、细胞活力、增殖、黏附及细胞周期蛋白D1(cyclinD1)、自噬和PI3K/AKT通路相关蛋白表达水平分别用实时荧光定量PCR(RT-qPCR)法、细胞计数试剂盒-8(CCK-8)、5-乙炔基-2'脱氧尿嘧啶核苷(EdU)试剂盒、细胞黏附实验及蛋白免疫印迹(WB)法测定。结果:miR-340 mimics组的miR-340表达水平显著高于mimics NC组(P<0.05),证明miR-340 mimics转染成功；与mimics NC组相比，miR-340 mimics组细胞活力、增殖能力、黏附能力、cyclinD1、人微管相关蛋白轻链3I(LC3I)及磷酸化(p)-PI3K、p-AKT蛋白表达水平显著下降(P<0.05),LC3II蛋白表达水平显著升高(P<0.05);与miR-340 mimics组相比，miR-340 mimics+抑制剂组上述指标变化趋势更明显(P<0.05),而miR-340 mimics+激活剂组的各指标变化趋势则相反(P<0.05)。结论:过表达miR-340或可通过调控PI3K/AKT信号通路抑制人胃癌AGS细胞增殖和黏附，促进细胞自噬。

关键词(KeyWords)： 胃癌;微小核糖核酸-340;磷脂酰肌醇3-激酶/丝苏氨酸蛋白激酶信号通路;增殖;自噬;黏附

Abstract:

Keywords:

基金项目(Foundation): 陕西省重点研发计划项目(2019SF-045)

作者(Author): 郭初阳,李冲,张海亮

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